방사선의학 웹진

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Abstract
Fluorescence imaging agents have recently received huge attention due to their important role in disease diagnostics. However, the intrinsic problems of these probes, such as complex synthetic routes and high molecular weight, remain challenging. Here, we developed novel phenaleno isoquinolinium-based fluorescent agents, Medical Fluorophores 37-41 (MF37-41), applicable to the quantitative and sensitive detection of sentinel lymph nodes (SLNs). These imaging agents showed no adverse effects on the proliferation of immune and normal cells and did not induce in vivo toxicity. In vivo fluorescence lifetime imaging demonstrated the accumulation of phenaleno isoquinolinium salts in the SLNs of nude mice within 15 min postinjection, consistent with our biodistribution findings. These results suggest that phenaleno isoquinolinium salts are feasible fluorescence imaging agents that can be used as potential lymphatic tracers.

Affiliations

Ye Ri Han  1 , Da-Sol Lee  2 , Sang Bong Lee  3 , Hui-Jeon Jeon  1 , Sijoon Lee  2 , Soo-Eun Sung  2 , Chang-Hee Lee  4 , Sung Jin Cho  1 , Kil Soo Kim  2   5 , Dong-Su Kim  6 , Yong Hyun Jeon  2   7
1 New Drug Development Center Daegu-Gyeongbuk Medical Innovation Foundation Daegu 700-721, South Korea.
2 Laboratory Animal Center Daegu-Gyeongbuk Medical Innovation Foundation Daegu 700-721, South Korea. jeon9014@gmail.com.
3 Vaccine Commercialization Center, Gyeongbuk Institute for Bio industry, 88 Saneopdanji-gil, pungsan-eup, Andong-si, Gyeongbuk, 33618, South Korea.
4 Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
5 College of Veterinary Medicine, Kyungpook National University, Daegu 41566, South Korea.
6 Therapeutics and Biotechnology Division, Korea Research Institute of Chemical Technology, 141 Gajeongro, Yuseong, Daejeon 31414, South Korea. dskim82@krict.re.kr.
7 Leading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University Hospital, Daegu, South Korea.