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Abstract
We introduce an efficient cell tracking imaging protocol using positron emission tomography (PET). Since macrophages are known to home and accumulate in tumor tissues and atherosclerotic plaque, we design a PET imaging protocol for macrophage cell tracking using aza-dibenzocyclooctyne-tethered PEGylated mesoporous silica nanoparticles (DBCO-MSNs) with the short half-life F-18-labeled azide-radiotracer via an in vivo strain-promoted alkyne azide cycloaddition (SPAAC) covalent labeling reaction inside macrophage cells in vivo. This PET imaging protocol for in vivo cell tracking successfully visualizes the migration of macrophage cells into the tumor site by the bioorthogonal SPAAC reaction of DBCO-MSNs with [18F]fluoropentaethylene glycolic azide ([18F]2) to form 18F-labeled aza-dibenzocycloocta-triazolic MSNs (18F-DBCOT-MSNs) inside RAW 264.7 cells. The tissue radioactivity distribution results were consistent with PET imaging findings. In addition, PET images of atherosclerosis in ApoE-/- mice fed a western diet for 30 weeks were obtained using the devised macrophage cell-tracking protocol.

Author information

Jeong HJ1, Yoo RJ2, Kim JK1, Kim MH2, Park SH1, Kim H1, Lim JW1, Do SH3, Lee KC2, Lee YJ4, Kim DW5.
1
Department of Chemistry and Chemical Engineering, Inha University, 100 Inharo, Namgu, Incheon, 22212, South Korea.
2
Department Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-706, South Korea.
3
Department of Clinical Pathology, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Seoul, South Korea.
4
Department Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-706, South Korea. Electronic address: yjlee@kirams.re.kr.
5
Department of Chemistry and Chemical Engineering, Inha University, 100 Inharo, Namgu, Incheon, 22212, South Korea. Electronic address: kimdw@inha.ac.kr.