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분자영상 및 방사화학
- 2017년 07월호
[Cancer Biother Radiopharm.] Development of 68Ga-SCN-DOTA-Capsaicin as an Imaging Agent Targeting Apoptosis and Cell Cycle Arrest in Breast Cancer.동국대/ 이준영,이상윤, 김상욱*
- 출처
- Cancer Biother Radiopharm.
- 등재일
- 2017 Jun
- 저널이슈번호
- 32(5):169-175. doi: 10.1089/cbr.2017.2186. Epub 2017 Jun 9.
- 내용
Abstract
68Ga-labeled capsaicin using a DOTA (1,4,7,10-tetraazocyclododecane-N,N',N″,N'″-tetraacetic acid) derivative [68Ga-SCN-Benzyl(Bn)-DOTA-capsaicin] was studied for the diagnosis of breast cancers, such as MCF-7 and SK-BR-3. The standard compound, 69Ga-SCN-Bn-DOTA-capsaicin, was also prepared and characterized by spectroscopic analysis. The binding affinity of 68Ga-SCN-Bn-DOTA-capsaicin was evaluated by using breast cancer cell lines (MCF-7, SK-BR-3) and colon cancer cell (CT-26); the biodistribution was carried out by using MCF-7-bearing nude mice, after which the positron emission tomography (PET) images were obtained at different time intervals (15-120 minutes). 68Ga-SCN-Bn-DOTA-capsaicin showed a cellular uptake of 0.93% Injected Dose (ID) after 30 minutes of incubation, whereas 68Ga-SCN-Bn-DOTA showed a lower uptake of 0.25% ID. The tumor-to-blood ID/g% ratios increased and were found to be 0.49, 0.22, and 0.77 for 15, 30, and 60 minutes, respectively. The small-animal PET study showed that the uptake of 68Ga-SCN-Bn-DOTA-capsaicin was higher in the tumor regions even at 30 minutes after injection. These results suggest that 68Ga-SCN-Bn-DOTA-capsaicin is a potential targeting agent for PET imaging of MCF-7.
Author information
Lee JY1, Lee SY1, Kim GG1,2, Hur MG1, Yang SD1, Park JH1, Kim SW2.
11 Radiation Instrumentation Research Division, Korea Atomic Energy Research Institute , Jeongeup, Republic of Korea.22 Department of Advanced Materials Chemistry, College of Science and Technology, Dongguk University , Gyeongju, Republic of Korea.
- 키워드
- 68Ga; anticancer; apoptosis; breast cancer; capsaicin; cell cycle arrest; positron emission tomography
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