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Abstract

Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis. However, at present, the effects of the metabolic labeling agents on cell metabolism and physiology are not known. To address this question, in this study, we analyzed the effects of cells treated with Ac4ManNAz through microarray analysis and analyses of membrane channel activity, individual bio-physiological properties, and glycolytic flux. According to the results, treatment with 50 μM Ac4ManNAz led to the reduction of major cellular functions, including energy generation capacity, cellular infiltration ability and channel activity. Interestingly, 10 μM Ac4ManNAz showed the least effect on cellular systems and had a sufficient labeling efficiency for cell labeling, tracking and proteomic analysis. Based on our results, we suggest 10 μM as the optimum concentration of Ac4ManNAz for in vivo cell labeling and tracking. Additionally, we expect that our approach could be used for cell-based therapy for monitoring the efficacy of molecule delivery and the fate of recipient cells. 

Author information

Han SS1, Lee DE2, Shim HE1, Lee S3, Jung T4, Oh JH5, Lee HA1, Moon SH4, Jeon J2,6, Yoon S5, Kim K3, Kang SW1,7.​

1Predictive Model Research Center, Korea Institute of Toxicology, Daejeon, Korea.2Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk, Korea.3Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea.4Department of Medicine, School of Medicine, Konkuk University, Seoul, Korea.5System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, Korea.6Department of Radiation Biotechnology and Applied Radioiostope Science, University of Science and Technology, Daejeon, Korea.7Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Korea.