글로벌 연구동향
방사선생물학
- 2016년 05월호
[Int J Radiat Oncol Biol Phys.] Site-Specific Phosphorylation of Ikaros Induced by Low-Dose Ionizing Radiation Regulates Cell Cycle Progression of B Lymphoblast Through CK2 and AKT Activation.방사선보건연구원/조성준, 양광희*
- 출처
- Int J Radiat Oncol Biol Phys.
- 등재일
- 2016 Apr 1
- 저널이슈번호
- 94(5):1207-18.
- 내용
Abstract
PURPOSE:
To determine how low-dose ionizing radiation (LDIR) regulates B lympho-proliferation and its molecular mechanism related with Ikaros, transcription factor.
METHODS AND MATERIALS:
Splenocytes and IM-9 cells were uniformly irradiated with various doses of a (137)Cs γ-source, and cell proliferation was analyzed. To determine the LDIR-specific phosphorylation of Ikaros, immunoprecipitation and Western blot analysis were performed. To investigate the physiologic function of LDIR-mediatied Ikaros phosphorylation, Ikaros mutants at phosphorylation sites were generated, and cell cycle analysis was performed.
RESULTS:
First, we found that LDIR enhances B lymphoblast proliferation in an Ikaros-dependent manner. Moreover, we found that LDIR elevates the phosphorylation level of Ikaros protein. Interestingly, we showed that CK2 and AKT are involved in LDIR-induced Ikaros phosphorylation and capable of regulating DNA binding activity of Ikaros via specific phosphorylation. Finally, we identified LDIR-specific Ikaros phosphorylation sites at S391/S393 and showed that the Ikaros phosphorylations at these sites control Ikaros's ability to regulate G1/S cell cycle progression.
CONCLUSION:
Low-dose ionizing radiation specifically phosphorylates Ikaros protein at Ser 391/393 residues to regulate cell cycle progression in B lymphoblast.
Author information
Cho SJ1, Kang H1, Kim MY2, Lee JE1, Kim SJ1, Nam SY1, Kim JY1, Kim HS1, Pyo S3, Yang KH4.
1KHNP Radiation Health Institute, Korea Hydro & Nuclear Power Co, Seoul, South Korea.
2Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan, South Korea.
3College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, South Korea.
4KHNP Radiation Health Institute, Korea Hydro & Nuclear Power Co, Seoul, South Korea. Electronic address: kwangheey@khnp.co.kr.
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