성균관대 / 김유진, 송지훈, 방석호*
Abstract
Background: High-energy irradiation eliminates cancer cells by destroying their genetic components. However, there are several side effects from doing this, such as fatigue, dermatitis, and hair loss, which remain obstacles to this treatment. Here, we propose a moderate method that uses low-energy white light from a light-emitting diode (LED) to selectively inhibit cancer cell proliferation without affecting normal cells.
Methods: The association between LED irradiation and cancer cell growth arrest was evaluated based on cell proliferation, viability, and apoptotic activity. Immunofluorescence, polymerase chain reaction, and western blotting were performed in vitro and in vivo to identify the metabolism related to the inhibition of HeLa cell proliferation.
Results: LED irradiation aggravated the defective p53 signaling pathway and induced cell growth arrest in cancer cells. Consequently, cancer cell apoptosis was induced by the increased DNA damage. Additionally, LED irradiation inhibited the proliferation of cancer cells by suppressing the MAPK pathway. Furthermore, the suppression of cancer growth by the regulation of p53 and MAPK was observed in cancer-bearing mice irradiated with LED.
Conclusions: Our findings suggest that LED irradiation can suppress cancer cell activity and may contribute to preventing the proliferation of cancer cells after medical surgery without causing side effects.
Affiliations
Yu-Jin Kim 1, Jihun Song 2, Dong-Hyun Lee 3, Soong Ho Um 3, Suk Ho Bhang 4
1School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea; Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
2School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea; Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Republic of Korea.
3School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
4School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address: sukhobhang@skku.edu.
편집위원
방사선 치료는 필수적인 항암요법이지만 탈모를 포함한 정상 세포 손상으로 인해 여러 가지 부작용이 발생한다. 본 연구는 LED의 저에너지 백색광을 활용하여 정상 세포에 영향을 주지 않고 암세포 특이적인 사멸을 유도할 수 있음을 확인하였다. LED 조사는 p53/MAPK 경로를 억제하여 세포사멸을 유도하였으며, 마우스 모델에서 LED 조사에 의한 암 성장 억제를 검증하였다. 본 연구는 암 치료 용도로서의 LED 활용 가능성을 시사하며, 신규 암 치료법에 대한 기반을 제시하였다는 점에 의의가 있다.
2023-07-05 14:35:16