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Abstract
BACKGROUND:
RAS GTPase-activating protein-binding protein (G3BP1) is an RNA-binding protein that is essential for assembling stress granules. Many functions related to the survival and progression of cancer have been reported. The current study aimed to investigate the role of G3BP1 in radio-sensitisation of cancer cells.

MATERIALS AND METHODS:
Radiation sensitivity and chemosensitivity were analysed in A549 and H460 cells transfected with G3BP1 siRNAs, and N-acetyl-L-cysteine (NAC) was used to elucidate the involvement of reactive oxygen species (ROS).

RESULTS:
G3BP1 depletion sensitised lung cancer cell lines to radiation, and the effect was related to ROS. G3BP1 depletion impaired the intracellular ROS scavenging system and NAC abolished the radiation-sensitive phenotypes caused by G3BP1 depletion.

CONCLUSION:
The study suggested G3BP1 as a promising target for radio- and chemosensitisation of lung cancer.

Author information

Cho E1, Than TT1, Kim SH1, Park ER2, Kim MY2, Lee KH2, Shin HJ3
1
Team of Radiation Convergence Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
2
Division of Radiation Biomedical Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
3
Team of Radiation Convergence Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea hjshin@kcch.re.kr.