글로벌 연구동향
핵의학
- 2022년 06월호
[Synapse.] Limited power of dopamine transporter mRNA mapping for predicting dopamine transporter availability부산대병원 / 박경준*
- 출처
- Synapse.
- 등재일
- 2022 Apr
- 저널이슈번호
- 76(5-6):e22226. doi: 10.1002/syn.22226. Epub 2022 Feb 13.
- 내용
Abstract
Dopamine transporters (DAT) are transmembrane proteins that translocate dopamine from the extracellular space into presynaptic neurons. We aimed to investigate the predictive power of DAT mRNA for DAT protein expression, measured using positron emission tomography (PET). We performed 18 F-FP-CIT PET scans in 35 healthy individuals. Binding potentials (BPND ) from the ventral striatum, caudate nucleus, putamen, and middle frontal, orbitofrontal, cingulate, parietal, and temporal cortices were measured. DAT gene expression data were obtained from the freely available Allen Human Brain Atlas derived from six healthy donors. The auto-correlation of PET-derived BPND s for DAT was intermediate (mean ρ2 = .66) with ρ2 ranging from .0811 to 1. However, the auto-correlation of mRNA expression was weak across the probes with a mean ρ2 of .09-.23. Cross-correlations between PET-derived BPND s and mRNA expression were weak with a mean ρ2 ranging from 0 to .22 across the probes. In conclusion, we observed weak associations between DAT mRNA expression and DAT availability in human brains. Therefore, DAT mRNA mapping may have only limited predictive power for DAT availability in humans. However, the difference in distribution of DAT mRNA and DAT protein may influence this limitation.Affiliations
Kyoungjune Pak 1 , Seongho Seo 2 , Myung Jun Lee 3 , Hyung-Jun Im 4 , Keunyoung Kim 1 , In Joo Kim 1
1 Department of Nuclear Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
2 Department of Electronic Engineering, Pai Chai University, Daejeon, Republic of Korea.
3 Department of Neurology, Pusan National University Hospital, Busan, Republic of Korea.
4 Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea.
- 키워드
- dopamine plasma membrane transport proteins; messenger RNA; positron emission tomography.
- 연구소개
- F18-FP-CIT PET에서 얻은 도파민전달체 (Dopamine transporter, DAT)의 protein expression과 Allen Human Brain Atlas에서 얻은 mRNA의 관련성에 대한 연구임. mRNA probe에 따라서 차이는 있으나, 전반적으로 약하거나 미미한 정도의 관련성이 있는 것으로 확인됨.
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